• 提示：詳情請(qǐng)下載說(shuō)明書(shū)。

• 介紹：

• 來(lái)源：牛血漿

• 性狀(以下信息僅供參考)：白色或類(lèi)白色無(wú)定形粉末或固體。

• 穩(wěn)定性：水溶液室溫下8h內(nèi)失活。遇熱、稀酸、堿、金屬等活力降低。

• 介紹：由動(dòng)物血漿分離制得的凝血酶原，再用凝血致活酶和氯化鈣激活而成。是一種蛋白水解酶，血漿中的可溶性纖維蛋白原在凝血酶的激活下，轉(zhuǎn)變成不溶性纖維蛋白網(wǎng)狀結(jié)構(gòu)，使血液凝固。纖維蛋白原有六條肽鏈組成，凝血酶的組成是從四條肽鏈的N端切斷一定特定的肽鍵（—Arg—Gly），放出兩個(gè)A肽（19肽）和兩個(gè)B肽（21肽），另二條N端為酷氨酸的肽鏈沒(méi)有變化。A肽和B肽切除后，減少了蛋白質(zhì)分子的負(fù)電荷，促進(jìn)了纖維蛋白分子的直線聚合和側(cè)向聚合，從而形成網(wǎng)狀結(jié)構(gòu)的纖維蛋白.

• 注意事項(xiàng)：

• 1、0.9%的nacl 保存時(shí)濃度要大于500U/ml；保存時(shí)分裝-20℃保存，不能反復(fù)凍融，復(fù)溶先以放到4℃緩沖1-2h，再拿到常溫使用。（溶液狀態(tài)儲(chǔ)存，活力會(huì)有所下降，酶屬于蛋白類(lèi)，無(wú)法避免，如果遇到酶活降低情況，可以降低稀釋倍數(shù)來(lái)達(dá)到之前的活性。）

• 2、纖維蛋白原（約2mg/ml）0.9ml，加凝血酶（10單位/ml）0.1ml，37℃凝固時(shí)間大約30秒；纖維蛋白原（約2mg/ml）0.9ml，加凝血酶（5單位/ml）0.1ml，37℃凝固時(shí)間大約50秒；溶液均用0.9%氯化鈉制備。 

• 3、1ku一支產(chǎn)品總質(zhì)量大約30mg左右，里面有酶蛋白，緩沖鹽和輔料等，單獨(dú)取用有可能會(huì)不均勻，建議整瓶溶解。僅供參考

• 儲(chǔ)存條件： -20℃

• 用途： 能促使纖維蛋白原轉(zhuǎn)化為纖維蛋白的酶。

• 注意：部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。

• 91. [5.4] Nan Lv et al."Enhanced-permeability delivery system for hydroxyl radical-responsive NIR-II fluorescence-monitored thrombolytic therapy."COLLOIDS AND SURFACES B-BIOINTERFACES.2025 Jan;245:114193

• 90. [10] Ke Gai et al."Development of Neural Cells and Spontaneous Neural Activities in Engineered Brain-Like Constructs for Transplantation."Advanced Healthcare Materials.2024 Sep;:2401419

• 89. [3.7] Xuewei Du et al."Ferricyanide-Mediated, Electrocatalytic Mechanism of Electrochemical Aptamer-Based Sensor Supports Ultrasensitive Analysis of Cardiac Troponin I in Clinical Samples."LANGMUIR.2024;40(34):18214–18224

• 88. [4.2] Fengmin Xiong et al."Fusion protein of FGF21 and elastin-like peptide improves wound healing in diabetic mice via inflammation modulation, collagen synthesis, and vascular network formation."EUROPEAN JOURNAL OF PHARMACOLOGY.2024 Nov;982:176953

• 87. [10.7] Lianqi Shan et al."Drug delivery under cover of erythrocytes extends drug half-life: A thrombolytic targeting therapy utilizing microenvironment-responsive artificial polysaccharide microvesicles."CARBOHYDRATE POLYMERS.2024 Nov;343:122505

• 86. [2.6] Si-Miao Yu et al."Chemoproteomic strategy identifies PfUCHL3 as the target of halofuginone."CHEMBIOCHEM.2024 Jun;:e202400269

• 85. [8.3] Xinyu Wang et al."Dual-Responsive Microsphere Based on Natural Sunflower Pollen for Hemostasis and Repair in Inflammatory Bowel Disease."ACS Applied Materials & Interfaces.2024;XXXX(XXX):XXX-XXX

• 84. [IF=4.4] Zhe Jiao et al."Highly-Selective fluorescent Fe3O4@PPy aptasensor."SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY".2024 Oct;318:124447

• 83. [IF=2.1] Hongwen Yang et al."Intrauterine infusion of platelet‐rich plasma improves fibrosis by transforming growth factor beta 1/Smad pathway in a rat intrauterine adhesion model."Reproductive Biology".2024 Jun;24:100882

• 82. [IF=6.1] Yaran Zhang et al."A simple, rapid and sensitive sandwich immunoassay based on poly(N-isopropylacrylamide) for the detection of alpha-fetoprotein."TALANTA".2024 Jul;274:125932

• 81. [IF=11.2] Caijie Yuan et al."Thrombotic microenvironment responsive crosslinking cyclodextrin metal-organic framework nanocarriers for precise targeting and thrombolysis."CARBOHYDRATE POLYMERS".2024 Jun;334:122058

• 80. [IF=24.1] Tan Suiyi et al."gp120-derived amyloidogenic peptides form amyloid fibrils that increase HIV-1 infectivity."Cellular & Molecular Immunology".2024 Mar;:1-16

• 79. [IF=2.5] Li Xidong et al."Purification and Characterization of a Novel Anticoagulant Peptide from Protein Hydrolysate of Eupolyphaga sinensis Walker."International Journal of Peptide Research and Therapeutics".2024 Mar;30(2):1-15

• 78. [IF=15.1] Xiangrong Yu et al."High Intensity Focused Ultrasound-Driven Nanomotor for Effective Ferroptosis-Immunotherapy of TNBC."Advanced Science".2024 Feb;:2305546

• 77. [IF=25] Xurui Liu et al."Magnetic soft microfiberbots for robotic embolization."Science Robotics".2024 Feb;9(87)

• 76. [IF=5.7] Gan Wang et al."Engineered three-dimensional bioactive scaffold for enhanced bone regeneration through modulating transplanted adipose derived mesenchymal stem cell and stimulating angiogenesis."Frontiers in Bioengineering and Biotechnology.2024; 12: 13425

• 75. [IF=8.2] Ruyu Wang et al."Danshensu inhibits SARS-CoV-2 by targeting its main protease as a specific covalent inhibitor and discovery of bifunctional compounds eliciting antiviral and anti-inflammatory activity."INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES.20

• 74. [IF=4.3] Zou Rong et al."Transistor-based immunosensor using AuNPs-Ab2-HRP enzyme nanoprobe for the detection of antigen biomarker in human blood."ANALYTICAL AND BIOANALYTICAL CHEMISTRY.2023 Nov;:1-11

• 73. [IF=5.7] Liu Jinying et al."Multi-signal aptasensor for thrombin detection based on catalytically active gold nanoparticles and fluorescent silicon quantum dots."MICROCHIMICA ACTA.2023 Nov;190(11):1-12

• 72. [IF=15.1] Ming Li et al."Distinguishable Colorimetric Biosensor for Diagnosis of Prostate Cancer Bone Metastases."Advanced Science.2023 Oct;10(32):2303159

• 71. [IF=8.2] Jingwen Tang et al."Highly absorbent bio-sponge based on carboxymethyl chitosan/poly-γ-glutamic acid/platelet-rich plasma for hemostasis and wound healing."INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES.2023 Jul;:125754

• 70. [IF=5.743] Zehao Wang et al."In-Situ Fabrication of Electroactive Cu2+-Trithiocyanate Complex and Its Application for Label-Free Electrochemical Aptasensing of Thrombin."Biosensors-Basel.2023 May;13(5):532

• 69. [IF=15.717] Shunli Wu et al."Ultrasound-triggered in situ gelation with ROS-controlled drug release for cartilage repair."Materials Horizons.2023 May;:

• 68. [0] Man Zhu et al."Employing a Redox Reporter-Modified Self-Assembly Monolayer in Electrochemical Aptamer-Based Sensors to Enable Calibration-Free Measurements."ACS Applied Bio Materials.2023;XXXX(XXX):XXX-XXX

• 67. [5.304] Zhiwei Liu et al."Hemin-catalyzed SI-RAFT polymerization for thrombin detection."MICROCHEMICAL JOURNAL.2023 Feb;:108521

• 66. [5.999] Ting Wang et al."Sodium alginate hydrogel containing platelet-rich plasma?for wound healing."COLLOIDS AND SURFACES B-BIOINTERFACES.2023 Feb;222:113096

• 65. [8.008] Shuang Xia et al."Paper-Based Distance Sensor for the Detection of Lipase via a Phase Separation-Induced Viscosity Change."ANALYTICAL CHEMISTRY.2022;XXXX(XXX):XXX-XXX

• 64. [3.21] Man Zhu et al."Incorporation of a Multi-Valent Aptamer into Electrochemical Biosensors to Achieve an Improved Performance for Thrombin Analysis in Blood Serum."ChemPlusChem.2022 Nov;87(11):e202200325

• 63. [IF=6.269] Jing Chen et al."A natural heparinoid from mollusc Meretrix lusoria: Purification, structural characterization, and antithrombotic evaluation."Current Research in Food Science.2022 Oct;:

• 62. [IF=5.561] Yuan Li et al."Characterization of a Nattokinase from the Newly Isolated Bile Salt-Resistant Bacillus mojavensis LY-06."Foods.2022 Jan;11(16):2403

• 61. [IF=10.383] Songzhi Xie et al."Bacteria-Templated and Dual Enzyme-Powered Microcapsule Motors To Promote Thrombus Penetration and Thrombolytic Efficacy."ACS Applied Materials & Interfaces.2022;14(33):37553–37565

• 60. [IF=4.927] Yunxiao Feng et al."Fluorescent Immunoassay with a Copper Polymer as the Signal Label for Catalytic Oxidation of O-Phenylenediamine."MOLECULES.2022 Jan;27(12):3675

• 59. [IF=2.464] Weiwei Luo et al."A sensitive electrochemiluminescent sensor chip based on the ssDNA-Ru(II) complex and aptamer for the determination of thrombin."LUMINESCENCE

• 58. [IF=2.891] Yunxiao Feng et al."A General, Label-Free and Homogeneous Electrochemical Strategy for Probing of Protease Activity and Screening of Inhibitor."Micromachines. 2022 May;13(5):803

• 57. [IF=2.162] Yajie Duan et al."Production, purification and characterization of a novel antithrombotic and anticoagulant serine protease from food grade microorganism Neurospora crassa."Preparative Biochemistry & Biotechnology. 2022 Jan 09

• 56. [IF=9.381] Fan Leng et al."Size-tunable and biodegradable thrombin-functionalized carboxymethyl chitin microspheres for endovascular embolization."Carbohyd Polym. 2022 Jun;286:119274

• 55. [IF=6.057] Ghazala Ashraf et al."Extension of duplex specific nuclease sensing application with RNA aptamer."Talanta. 2022 May;242:123314

• 54. [IF=6.475] Juanjuan Du et al."Insight into the mechanism of myosin-fibrin gelation induced by non-disulfide covalent cross-linking."Food Res Int. 2022 Jun;156:111168

• 53. [IF=13.281] Ting Li et al.Engineered Platelet-Based Micro/Nanomotors for Cancer Therapy.Small.2021 Nov 05

• 52. [IF=4.142] Qu Fei et al."Ratiometric detection of alkaline phosphatase based on aggregation-induced emission enhancement."Anal Bioanal Chem. 2019 Nov;411(28):7431-7440

• 51. [IF=4.952] Juanjuan Du et al."The effect of fibrin on rheological behavior, gelling properties and microstructure of myofibrillar proteins."Lwt Food Sci Technol. 2022 Jan;153:112457

• 50. [IF=5.118] ZhenXing Du et al."Isolation and Characterization of a Heparin-Like Compound with Potent Anticoagulant and Fibrinolytic Activity from the Clam Coelomactra antiquata."Mar Drugs. 2020 Jan;18(1):6

• 49. [IF=5.89] Bingchuan Lu et al."Rapid Fabrication of Cell-Laden Microfibers for Construction of Aligned Biomimetic Tissue."Front Bioeng Biotech. 2020; 8: 610249

• 48. [IF=6.057] Yu Xin Liu et al."DNA nanosheet as an excellent fluorescence anisotropy amplification platform for accurate and sensitive biosensing."Talanta. 2020 May;211:120730

• 47. [IF=6.558] Wenjing Qi et al."Electrochemiluminescence resonance energy transfer immunoassay for alkaline phosphatase using p-nitrophenyl phosphate as substrate."Anal Chim Acta. 2020 Feb;1097:71

• 46. [IF=6.843] Yuting Shi et al."Preparation and application of quick hemostatic gauze based on biomimetic mineralized thrombin."Biomater Sci-Uk. 2021 Sep;9(18):6098-6107

• 45. [IF=7.514] Xin Zheng et al."Identification of the functional food ingredients with antithrombotic properties via virtual screen and experimental studies."Food Chem. 2021 Nov;362:130237

• 44. [IF=7.727] Xing Pei et al."Local delivery of cardiac stem cells overexpressing HIF-1α promotes angiogenesis and muscular tissue repair in a hind limb ischemia model."J Control Release. 2020 Jun;322:610

• 43. [IF=9.381] Zhenghui Shi et al."Puff pastry-like chitosan/konjac glucomannan matrix with thrombin-occupied microporous starch particles as a composite for hemostasis."Carbohyd Polym. 2020 Mar;232:115814

• 42. [IF=13.273] Zhenghui Shi et al."Targeted delivery of hemostats to complex bleeding wounds with magnetic guidance for instant hemostasis."Chem Eng J. 2022 Jan;427:130916

• 41. [IF=1.134] Song-Bai ZHANG et al."Highly Sensitive Fluorescent Aptasensor for Thrombin Detection Based on Competition Triggered Rolling Circle Amplification."Chinese J Anal Chem. 2015 Nov;43:1688

• 40. [IF=2.985] Hongwei Wang et al."Anti-complementary activity of two homogeneous polysaccharides from Eclipta prostrata."Biochem Bioph Res Co. 2017 Nov;493:887

• 39. [IF=3.361] Lingmei Lv et al."Preparation and characterization of a chitin/platelet-poor plasma composite as a hemostatic material."Rsc Adv. 2016 Oct;6(98):95358-95368

• 38. [IF=4.464] Fang Yuan et al."Split photoelectrochemistry for the immunoassay of α-fetoprotein based on graphitic carbon nitride."J Electroanal Chem. 2016 Dec;783:226

• 37. [IF=6.232] Guang-Li Wang et al."Fluorescent aptamer-based assay for thrombin with large signal amplification using peroxidase mimetics."Microchim Acta. 2016 Feb;183(2):765-771

• 36. [IF=6.558] Yuanhong Xu et al."Multifunctional solid-state electrochemiluminescent chemosensors and aptasensor with free-standing active sites based on task-specific pyrene-terminated polymers via RAFT polymerization."Anal Chim Acta. 2018 Dec;1039:31

• 35. [IF=6.785] Lu-Yi Jin et al."Versatile and Amplified Biosensing through Enzymatic Cascade Reaction by Coupling Alkaline Phosphatase in Situ Generation of Photoresponsive Nanozyme."Anal Chem. 2015;87(20):10429–10436

• 34. [IF=7.1] Xin Fang et al."Tuning surface states to achieve the modulated fluorescence of carbon dots for probing the activity of alkaline phosphatase and immunoassay of α-fetoprotein."Sensor Actuat B-Chem. 2018 Mar;257:620

• 33. [IF=7.1] Ping Li et al."A novel strategy for amplified probing versatile biomolecules through a photoswitchable biocatalytic cascade."Sensor Actuat B-Chem. 2018 Jun;262:110

• 32. [IF=7.46] Hong Wang et al."Ferricyanide stimulated cathodic photoelectrochemistry of flower-like bismuth oxyiodide under ambient air: A general strategy for robust bioanalysis."Sensor Actuat B-Chem. 2019 Jun;288:683

• 31. [IF=7.79] Yaping Zhong et al."Water-soluble MoS2 quantum dots for facile and sensitive fluorescence sensing of alkaline phosphatase activity in serum and live cells based on the inner filter effect."Nanoscale. 2018 Nov;10(45):21298-21306

• 30. [IF=8.198] Qing Li et al."Biodegradable Microporous Starch with Assembled Thrombin for Rapid Induction of Hemostasis."Acs Sustain Chem Eng. 2019;7(10):9121–9132

• 29. [IF=9.594] Fushuang Niu et al."Electrochemically generated green-fluorescent N-doped carbon quantum dots for facile monitoring alkaline phosphatase activity based on the Fe3+-mediating ON-OFF-ON-OFF fluorescence principle."Carbon. 2018 Feb;127:340

• 28. [IF=10.257] Xue–Lian Hu et al."Switchable fluorescence of gold nanoclusters for probing the activity of alkaline phosphatase and its application in immunoassay."Biosens Bioelectron. 2016 Mar;77:666

• 27. Tian, Li, Wenke Zhou, and Youhong Zhang. "Construction of a genetically engineered strain of nattokinase and assessment of its fibrinolytic activity." African Journal of Microbiology Research 13.27 (2019): 488-499.https://doi.org/10.5897/AJMR2019.9181

• 26. Ping, et al. "A novel strategy for amplified probing versatile biomolecules through a photoswitchable biocatalytic cascade." Sensors and Actuators, B. Chemical 262(2018):110-117.

• 25. Wang, Hongwei, et al. "Anti-complementary activity of two homogeneous polysaccharides from Eclipta prostrata." Biochemical and biophysical research communications 493.2 (2017): 887-893.https://doi.org/10.1016/j.bbrc.2017.09.126

• 24. Wang, Guang-Li, et al. "Fluorescent aptamer-based assay for thrombin with large signal amplification using peroxidase mimetics."?Microchimica Acta?183.2 (2016): 765-771.https://doi.org/10.1007/s00604-015-1703-5

• 23. Liu, Yu Xin, et al. "DNA nanosheet as an excellent fluorescence anisotropy amplification platform for accurate and sensitive biosensing." Talanta 211 (2020): 120730.https://doi.org/10.1016/j.talanta.2020.120730

• 22. 龍繼艷,戴明,鐘瑤,李夢(mèng)鑫,蔣詠梅.納豆發(fā)酵原料篩選及其功能產(chǎn)品的開(kāi)發(fā)[J].生物化工,2020,6(06):47-50.

• 21. 張立強(qiáng),冉茂芳,王松濤,劉光錢(qián),馬卓,任劍波,沈才洪,許濤.瀘州老窖大曲中高產(chǎn)纖溶酶菌株的分離及鑒定[J].釀酒科技,2021(03):17-24.

• 20. 王常瞵,劉國(guó)飛,向澤棟,董萍萍,趙紅金,高鵬,代龍.仿生提取法研究螞蟥不同部位體外抗凝活性[J].山東科學(xué),2020,33(04):13-17.

• 19. 李秀涼,李倩文,朱玉,韓曉云,孫慶申,宋永.納豆芽孢桿菌發(fā)酵菜用大豆中納豆激酶的提取及其性質(zhì)分析[J].中國(guó)釀造,2020,39(12):106-112.

• 18. 吳雄, 夏建開(kāi), 苗艷麗,等. 星點(diǎn)設(shè)計(jì)-效應(yīng)面法優(yōu)化復(fù)合酶解酪蛋白制備抗凝血肽的工藝研究[J]. 藥學(xué)研究, 2019, 038(006):P.336-342.

• 17. 王帥, 郭巧生, 史紅專,等. 短期添加17β-E2對(duì)越冬螞蟥生長(zhǎng)和性腺發(fā)育的影響.

• 16. 蘆鑫, 郭巧生, 史紅專,等. 外源甲基睪酮對(duì)自然越冬螞蟥生長(zhǎng)及性腺發(fā)育的影響.

• 15. 張旦旦, 竇文芳, 趙軍,等. 重組瑞替普酶在畢赤酵母中的表達(dá)[J]. 微生物學(xué)通報(bào), 2017(9).

• 14. 錢(qián)澤棟,張佑紅,盧育兵,朱麗娜,田莉.納豆菌的分離篩選與鑒定[J].化學(xué)與生物工程,2018,35(08):41-44.

• 13. 趙謀明,鄒穎,林戀竹,吳見(jiàn).納豆菌液態(tài)發(fā)酵蕎麥產(chǎn)納豆激酶及其代謝特性分析[J].食品科學(xué),2019,40(04):178-185.

• 12. 張星燦.納豆激酶在腐乳儲(chǔ)藏條件下穩(wěn)定性初探[J].中國(guó)調(diào)味品,2015,40(02):72-76+80.

• 11. 何夢(mèng)雪, 李澤友. 海南菲牛蛭抗凝血酶成分的提取與SDS-PAGE電泳測(cè)定[J]. 熱帶生物學(xué)報(bào), 2019, 010(002):204-207.

• 10. 張科衛(wèi), 鐘雨佶, 曹虹虹,等. 水蛭炮制前后質(zhì)量分析[J]. 中成藥, 2018, 40(10):134-139.

• 9. 王正俊, 李博, 郭立瑋,等. 影響胃蛋白酶和胰蛋白酶纖溶活性因素初探及其纖溶活性失活研究[J]. 中草藥, 2016, 47(01):56-66.

• 8. 王立, 張坤, 鄒燁,等. 超聲輔助提取對(duì)雞肝蛋白結(jié)構(gòu)及功能特性的影響[J]. 江蘇農(nóng)業(yè)學(xué)報(bào), 2018, 034(005):1153-1160.

• 7. 蔣曉煌,蔣孟良,賀衛(wèi)和,曾婷,劉瑩,孟珠,趙梓含,鄧瀟瀟.膠艾湯不同炮制組方對(duì)動(dòng)物子宮收縮、凝血酶原時(shí)間與纖溶活性的影響[J].中國(guó)醫(yī)院藥學(xué)雜志,2017,37(09):835-838.

• 6. 張松柏, 鄭麗英, 胡霞,等. 基于競(jìng)爭(zhēng)觸發(fā)滾環(huán)擴(kuò)增的熒光適配體傳感器高靈敏檢測(cè)凝血酶[J]. 分析化學(xué), 2015, 000(011):1688-1694.

• 5. 林陳水 陳微微. 分泌型水蛭素Ⅱ基因工程菌的構(gòu)建[J]. 浙江工業(yè)大學(xué)學(xué)報(bào) 2014 42(1):50-53.

• 4. 王常瞵 劉國(guó)飛 向澤棟 等. 仿生提取法研究螞蟥不同部位體外抗凝活性[J]. 山東科學(xué) 2020(4):13-17.

• 3. 田莉 盧軼男 朱建 等. 產(chǎn)納豆激酶的枯草芽孢桿菌基因工程菌發(fā)酵條件的響應(yīng)面優(yōu)化[J]. 武漢工程大學(xué)學(xué)報(bào) 2018 v.40;No.209(06):33-40.

• 2. 尹夢(mèng)雯 周倩 雷嬋 等. 產(chǎn)纖溶酶乳酸菌篩選及安全性評(píng)估[J]. 食品與機(jī)械 2017 033(010):28-33.

• 1. 白景芝 劉永杰. IL-6 RF ACCP及凝血指標(biāo)在冠心病合并類(lèi)風(fēng)濕關(guān)節(jié)炎患者中的表達(dá)及意義[J]. 中國(guó)衛(wèi)生工程學(xué) 2020 v.19(03):142-144.

輸入產(chǎn)品批號(hào)：

本計(jì)算器可幫助您計(jì)算出特定溶液中溶質(zhì)的質(zhì)量、溶液濃度和體積之間的關(guān)系，公式為：

質(zhì)量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *