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• 介紹：

       為重組 E. coli 菌株，其基因組中含有來(lái)源于 Thermusaquaticus YTI 中克隆的 Taq DNA polymerase 基因。本產(chǎn)品是一種熱穩(wěn)定性酶，分子量大約為 94kDa。它與天然 Tag DNA 聚合酶有相同的功能。在 Mg 2+存在的條件下可催化核苷酸沿 5’→3’方向發(fā)生聚合反應(yīng)，形成雙鏈 DNA；同時(shí)它還具有 5’ →3’外切酶活性。

   

      使用本品擴(kuò)增得到的 PCR 產(chǎn)物的 3´端附有一個(gè)"A"堿基，因此可直接克隆于 T-Vector 中。

   

  活性定義：在 74℃條件下，30 分鐘內(nèi)催化 10nmol 的 dNTP 摻入酸不溶性沉淀物所需要的酶量定義為一個(gè)活性單位。

   

  貯存條件： 20 mM Tris-HC（l pH 8.0，25℃），100 mM KCl，0.1 mM EDTA，1 mM DTT，0.5% NP-40 ，0.5% Tween -20 ，50%Glycerol 

   

  反應(yīng)條件： 50mM KCl， 10 mM Tris-HCl (Ph8.6，25℃)， 0.1% Triton X-100 ，1.5mM MgCl2. 

   

  質(zhì)量保證：本品經(jīng) PCR 驗(yàn)證，活性測(cè)定，內(nèi)切核酸酶/缺刻酶測(cè)定和 SDS-PAGE 純度鑒定。保證產(chǎn)品的質(zhì)量穩(wěn)定可靠。

   

  使用注意：除了酶活性喪失外，下列因素也可能導(dǎo)致擴(kuò)增失敗

   

  （1）模板太多或太少；（2）樣品中存在 Mg 2+ 絡(luò)合劑，導(dǎo)致 Mg 2+實(shí)際濃度過(guò)低；(3) PCR 儀溫度不準(zhǔn)； (4) 臨床來(lái)源的樣品中含有未知的 Taq 酶抑制劑； (5) 引物部分降解；(6) dNTP 部分降解；(7) Taq 酶用量太大等。

   

   建議：每 50 微升反應(yīng)體系中，酶用量為 0.5-1 微升最好，酶量少可能擴(kuò)增效率低下，使用時(shí)請(qǐng)注意! 

   

  用途： 

   1) 常規(guī) PCR 鑒定。

   2) 小片段目標(biāo)基因克?。ㄍ扑]小于 500bp）。

   3) 標(biāo)記 DNA 3’-末端。

   4) 平端 PCR 產(chǎn)物加“A”。

• 儲(chǔ)存條件： -20℃

• 注意：部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。

• 26. [5.561] Li, Yanqi et al."Contribution of microRNA-30d to the prevention of the thyroid cancer occurrence and progression: mechanism and implications."APOPTOSIS.2023 Jan;:1-18

• 25. [IF=3.911] Wu Gang et al."Overexpression of ORX or MCH Protects Neurological Function Against Ischemic Stroke."Neurotox Res. 2022 Jan;:1-12

• 24. [IF=3.448] Rong-Chuan Yue et al."Calpain silencing alleviates myocardial ischemia-reperfusion injury through the NLRP3/ASC/Caspase-1 axis in mice."Life Sci. 2019 Sep;233:116631

• 23. [IF=3.673] Qin Liu et al."Downregulation of microRNA-196a inhibits stem cell self-renewal ability and stemness in non-small-cell lung cancer through upregulating GPX3 expression."Int J Biochem Cell B. 2019 Oct;115:105571

• 22. [IF=3.996] Zhiyan Ruan et al."Shikonin-mediated PD-L1 degradation suppresses immune evasion in pancreatic cancer by inhibiting NF-κB/STAT3 and NF-κB/CSN5 signaling pathways."Pancreatology. 2021 Apr;21:630

• 21. [IF=4.06] Xianghui Zhang et al."MiR-199a-5p inhibition protects cognitive function of ischemic stroke rats by AKT signaling pathway."Am J Transl Res. 2020; 12(10): 6549–6558

• 20. [IF=4.238] Hong-Cheng Luo et al."Role of long noncoding RNA MEG3/miR-378/GRB2 axis in neuronal autophagy and neurological functional impairment in ischemic stroke."J Biol Chem. 2020 Oct;295(41):14125-14139

• 19. [IF=4.932] Jing Wang et al."MicroRNA-532-5p upregulation protects neurological deficits after ischemic stroke through inhibition of BTB and CNC homology 1."Int Immunopharmacol. 2021 Nov;100:108003

• 18. [IF=4.966] Na-Na Zhu et al."Autologous blood transfusion stimulates wound healing in diabetic mice through activation of the HIF‐1α pathway by improving the blood preservation solution."Faseb J. 2020 May;34(5):6038-6054

• 17. [IF=5.241] Ma Yu-Shui et al."Exosomal microRNA-15a from mesenchymal stem cells impedes hepatocellular carcinoma progression via downregulation of SALL4."Cell Death Discov. 2021 Aug;7(1):1-11

• 16. [IF=5.546] Ying Cai et al."Functional suppression of Epiregulin impairs angiogenesis and aggravates left ventricular remodeling by disrupting the extracellular‐signal‐regulated kinase1/2 signaling pathway in rats after acute myocardial infarction."J Cell Physiol. 20

• 15. [IF=5.59] Mao Lei et al."Elevation of miR-146a Inhibits BTG2/BAX Expression to Ameliorate Postoperative Cognitive Dysfunction Following Probiotics (VSL#3) Treatment."Mol Neurobiol. 2021 Jul;58(7):3457-3470

• 14. [IF=5.736] Minghuan Mao et al."OCT3/4 enhances tumor immune response by upregulating the TET1-dependent NRF2/MDM2 axis in bladder cancer."Genomics. 2021 Jul;113:2122

• 13. [IF=7.2] Yu-Shui Ma et al."M2 macrophage-derived exosomal microRNA-155-5p promotes the immune escape of colon cancer by downregulating ZC3H12B."Mol Ther-Oncolytics. 2021 Mar;20:484

• 12. [IF=2.939] Xue Yu-Long et al."Silencing of STAT4 Protects Against Autoimmune Myocarditis by Regulating Th1/Th2 Immune Response via Inactivation of the NF-κB Pathway in Rats."Inflammation. 2019 Aug;42(4):1179-1189

• 11. [IF=2.991] Luo Xiao-Yan et al."OSMR gene effect on the pathogenesis of chronic autoimmune Urticaria via the JAK/STAT3 pathway."Mol Med. 2018 Dec;24(1):1-13

• 10. [IF=3.098] Zhi?Qiang Zhou et al."Adipose extracellular matrix promotes skin wound healing by inducing the differentiation of adipose?derived stem cells into fibroblasts."Int J Mol Med. 2019 Feb;43(2):890-900

• 9. [IF=3.533] Liang-Liang Chen et al."SDC4 Gene Silencing Favors Human Papillary Thyroid Carcinoma Cell Apoptosis and Inhibits Epithelial Mesenchymal Transition via Wnt/β-Catenin Pathway."Mol Cells. 2018 Sep 30; 41(9): 853–867

• 8. [IF=4.522] Zhen-Wei Ge et al."IRAK3 gene silencing prevents cardiac rupture and ventricular remodeling through negative regulation of the NF‐κB signaling pathway in a mouse model of acute myocardial infarction."J Cell Physiol. 2019 Jul;234(7):11722-11733

• 7. [IF=4.522] Ru‐Xue Bai et al."Repression of TXNIP–NLRP3 axis restores intestinal barrier function via inhibition of myeloperoxidase activity and oxidative stress in nonalcoholic steatohepatitis."J Cell Physiol. 2019 May;234(5):7524-7538

• 6. [IF=4.522] Hong-Cheng Fang et al."KRT1 gene silencing ameliorates myocardial ischemia–reperfusion injury via the activation of the Notch signaling pathway in mouse models."J Cell Physiol. 2019 Apr;234(4):3634-3646

• 5. [IF=6.508] Shi Chen et al."Silencing of long noncoding RNA LINC00958 prevents tumor initiation of pancreatic cancer by acting as a sponge of microRNA-330-5p to down-regulate PAX8."Cancer Lett. 2019 Apr;446:49

• 4. Yue, Rong-Chuan, et al. "Calpain silencing alleviates myocardial ischemia-reperfusion injury through the NLRP3/ASC/Caspase-1 axis in mice." Life sciences 233 (2019): 116631.https://doi.org/10.1016/j.lfs.2019.116631

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• 2. Liang, Tian-Song, et al. "MicroRNA-506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the Wnt/β-catenin signaling pathway by down-regulating LHX2."?Journal of experimental & clinical cancer research?38.1 (2019)

• 1. Tian, Y, Shen, L, Li, F, Yang, J, Wan, X, Ouyang, M. Silencing of RHEB inhibits cell proliferation and promotes apoptosis in colorectal cancer cells via inhibition of the mTOR signaling pathway. J Cell Physiol. 2019; 235: 442– 453. https://doi.org/10.1002/

輸入產(chǎn)品批號(hào)：

本計(jì)算器可幫助您計(jì)算出特定溶液中溶質(zhì)的質(zhì)量、溶液濃度和體積之間的關(guān)系，公式為：

質(zhì)量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *