• 介紹：
  溴化十六烷基三甲胺：CTAB是一種陽(yáng)離子去污劑，具有從低離子強(qiáng)度的溶液中沉淀核酸和酸性多聚糖的特性，在這種條件下，蛋白質(zhì)和中性多聚糖仍留在溶液里，在高離子強(qiáng)度的溶液里，與蛋白質(zhì)和大多數(shù)酸性多聚糖以外的多聚糖形成復(fù)合物，只是不能沉淀核酸。因此，CTAB可以用于從大量產(chǎn)生粘多糖的有機(jī)體如植物以及某些革蘭氏陰性菌(包括E.coli的某些株)中制備純化DNA 。

  CTAB原理：CTAB是一種陽(yáng)離子去污劑，具有從低離子強(qiáng)度的溶液中沉淀核酸和酸性多聚糖的特性，在這種條件下，蛋白質(zhì)和中性多聚糖仍留在溶液里，在高離子強(qiáng)度的溶液里，能夠與蛋白質(zhì)和大多數(shù)酸性多聚糖以外的多聚糖形成復(fù)合物，只是不能沉淀核酸，從而將核酸分離出來(lái)。

  CTAB使用方法：

  1.提取DNA配方：

  CTAB 4g

  NaCl 16.364 g

  1M Tris-HCl 20 mL( PH8.0)

  冷卻后0.2-1%2-巰基乙醇（400ul）氯仿-異戊醇（24：1）：先加96ml氯仿，再加4ml異戊醇，搖勻即可。

  2.提取RNA配方

  2% CTAB（W/V）

  2%聚乙烯吡咯烷酮PVP（W/V）

  100 mM Tris-HCl（pH8.0,DEPC處理的水配制）

  25mM EDTA

  由于在高溫滅菌條件下，Tris-HCl要和DEPC發(fā)生反應(yīng)，所以配RNA提取緩沖液時(shí)直接用DEPC 處理的水

  CTAB使用注意事項(xiàng)：具有刺激性，若吸入、攝入或皮膚吸收，會(huì)對(duì)人體有害。使用時(shí)要戴合適的手套和防護(hù)眼鏡（或在通風(fēng)櫥操作）。避免吸入其粉塵。

• 熔點(diǎn)： 248-251℃

• 外觀： 白色結(jié)晶

• 溶解性： Souble  in  EtOH

• 敏感性： 易吸潮

• 儲(chǔ)存條件： RT

• 用途： 催化劑,乳化劑,消毒劑,殺菌基,抗靜電劑等。比色測(cè)定或光度測(cè)定銻和錫。腸內(nèi)葡萄糖吸收抑制劑。芳烴還原和烯環(huán)氧化時(shí)的相轉(zhuǎn)移催化劑。

• 注意：部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。

• 36. [IF=4.5] Zhang Mengmeng et al."Synergistic adsorption–photocatalytic removal toward levofloxacin and methylene blue by silver phosphate/strontium ferrite/graphene oxide composite under visible light irradiation."JOURNAL OF MATERIALS SCIENCE".2024 May;:1-21

• 35. [IF=5] Ling Li et al."Fabrication of One-Dimensional Polypyrrole Nanochains for a High-Efficiency Electromagnetic Wave Absorber with a Low Filler Loading."ACS Applied Polymer Materials".2024;XXXX(XXX):XXX-XXX

• 34. [IF=10] Huayuan Chen et al."Gastroretentive Raft Forming System for Enhancing Therapeutic Effect of Drug-Loaded Hollow Mesoporous Silica on Gastric Ulcers."Advanced Healthcare Materials".2024 May;:2400566

• 33. [IF=7] Wanfei Yu et al."A facile two-step method to construct environmental-friendly Janus coconut wood membrane for oil/water separation."Journal of Water Process Engineering.2023 Dec;56:104434

• 32. [IF=5.9] Lihua Yang et al."Visual Detection of Mercaptan Gases Using Silver Nanoparticles-Based Colorimetric Sensor Array."ACS Applied Nano Materials.2023;XXXX(XXX):XXX-XXX

• 31. [IF=4.7] Wanru Sun et al."Multifunctional Oxygen-Generating Nanoflowers for Enhanced Tumor Therapy."ACS Applied Bio Materials.2023;XXXX(XXX):XXX-XXX

• 30. [IF=3.4] Shu-ran Qin et al."Qualitative analysis on the transdermal absorption from the dichloromethane extract of the Sambucus adnata wall. based on UPLC-Q-Exactive Orbitrap/MS."JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS.2023 Sep;234:115509

• 29. [IF=3.8] Liu Siyuan et al."Improving rhamnolipids production using fermentation-foam fractionation coupling system: cell immobilization and waste frying oil emulsion."BIOPROCESS AND BIOSYSTEMS ENGINEERING.2023 Jun;:1-20

• 28. [IF=6] Shujuan Chen et al."Development of a green simple molecularly imprinted nanoprobe for rapid determination of trace thiamphenicol residual in animal derived foods."LWT-FOOD SCIENCE AND TECHNOLOGY.2023 Jul;184:115026

• 27. [IF=8.025] Yingying Xu et al."Catalytic hydrogenation of levulinic acid to γ-valerolactone over lignin-metal coordinated carbon nanospheres in water."INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES.2023 Jun;240:124451

• 26. [IF=8.025] Zhicheng Liu et al."Photothermal-accelerated urease-powered human serum albumin nanomotor for rapid and efficient photothermal and photodynamic cancer combination therapy."INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES.2023 Jun;240:124486

• 25. [0] Na Zhang et al."Improved energy storage property in polyvinylidene fluoride-based multilayered composite regulated by oriented carbon nanotube@SiO2 nanowires."IET Nanodielectrics.2023 Mar;:

• 24. [6.371] Tianyu Guo et al."Engineering shape of BiOCl nanosheets with improved visible-light response for superior photocatalytic degradation of Rhodamine B."JOURNAL OF ALLOYS AND COMPOUNDS.2023 Jul;948:169586

• 23. [7.129] Yuqian Jia et al."Disinfectants facilitate the transformation of exogenous antibiotic resistance genes via multiple pathways."ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY.2023 Mar;253:114678

• 22. [IF=9.231] Ximo Wang et al."Ultra-sensitive detection of streptomycin in foods using a novel SERS switch sensor fabricated by AuNRs array and DNA hydrogel embedded with DNAzyme."FOOD CHEMISTRY.2022 Nov;393:133413

• 21. [IF=5.645] Zelin Wang et al."A sustainable and integrated natural surfactant mediated microwave-assisted extraction technique enhances the extraction of phytochemicals from plants."INDUSTRIAL CROPS AND PRODUCTS. 2022 Sep;184:115043

• 20. [IF=3.125] Chuying Zhang et al."Study on structural and functional properties of porous SiO2 core-shell construction/polyethylene nanocomposites with enhanced interfacial interaction."Journal Of Applied Polymer Science. 2022 Jan 04

• 19. [IF=8.128] Yang Wang et al."Pillar[5]arene based glyco-targeting nitric oxide nanogenerator for hyperthermia-induced triple-mode cancer therapy."J Colloid Interf Sci. 2022 Jun;615:386

• 18. [IF=4.098] Meng Zhang et al."Assembly of gold nanorods with L-cysteine reduced graphene oxide for highly efficient NIR-triggered photothermal therapy."Spectrochim Acta A. 2022 Feb;266:120458

• 17. [IF=5.373] Han Yan et al."A label-free electrochemical immunosensing platform based on PEI-rGO/Pt@Au NRs for rapid and sensitive detection of zearalenone."Bioelectrochemistry. 2022 Feb;143:107955

• 16. [IF=5.833] He Baoshan et al."An amperometric zearalenone aptasensor based on signal amplification by using a composite prepared from porous platinum nanotubes, gold nanoparticles and thionine-labelled graphene oxide."Microchim Acta. 2019 Jun;186(6):1-10

• 15. [IF=7.46] Jiawen Li et al."Dual mode competitive electrochemical immunoassay for dibutyl phthalate detection based on PEI functionalized nitrogen doped graphene-CoSe2/gold nanowires and thionine-Au@Pt core-shell."Sensor Actuat B-Chem. 2021 Mar;331:129401

• 14. [IF=7.46] Baoshan He et al."A “signal-on” voltammetric aptasensor fabricated by hcPt@AuNFs/PEI-rGO and Fe3O4NRs/rGO for the detection of zearalenone."Sensor Actuat B-Chem. 2019 Jul;290:477

• 13. [IF=7.92] Xinqiang Feng et al."Is hydrolysis a bad news for p-xylene production from 2,5-dimethylfuran and ethylene? Mechanism investigation into the role of acid strength during 2,5-hexanedione conversion."J Catal. 2021 Sep;401:214

• 12. [IF=7.991] Ming Lu et al."A polyethyleneimine reduced graphene oxide/gold nanocubes based electrochemical aptasensor for chloramphenicol detection using single-stranded DNA-binding protein."Mater Design. 2021 Feb;199:109409

• 11. [IF=8.116] Giorgia Croppi et al."Discovery of an Inhibitor for Bacterial 3-Mercaptopyruvate Sulfurtransferase that Synergistically Controls Bacterial Survival."Cell Chem Biol. 2020 Dec;27:1483

• 10. [IF=8.131] Jiawei Liu et al."Defense and inhibition integrated mesoporous nanoselenium delivery system against tomato gray mold."Environ Sci-Nano. 2020 Jan;7(1):210-227

• 9. [IF=13.273] Baoshan He et al."Nb.BbvCI powered DNA walking machine-based Zr-MOFs-labeled electrochemical aptasensor using Pt@AuNRs/Fe-MOFs/PEI-rGO as electrode modification material for patulin detection."Chem Eng J. 2021 Feb;405:126642

• 8. Baoshan He, Xiaohai Yan, A “signal-on” voltammetric aptasensor fabricated by hcPt@AuNFs/PEI-rGO and Fe3O4NRs/rGO for the detection of zearalenone, Sensors and Actuators B: Chemical, Volume 290, 2019, Pages 477-483, ISSN 0925-4005, https://doi.org/10.1016/j

• 7. Baoshan He, Xiaoze Dong, Nb.BbvCI powered DNA walking machine-based Zr-MOFs-labeled electrochemical aptasensor using Pt@AuNRs/Fe-MOFs/PEI-rGO as electrode modification material for patulin detection, Chemical Engineering Journal, Volume 405, 2021, 126642,

• 6. He, B., Yan, X. An amperometric zearalenone aptasensor based on signal amplification by using a composite prepared from porous platinum nanotubes, gold nanoparticles and thionine-labelled graphene oxide. Microchim Acta 186, 383 (2019). https://doi.org/10.1

• 5. He, B., Yan, X. An amperometric zearalenone aptasensor based on signal amplification by using a composite prepared from porous platinum nanotubes, gold nanoparticles and thionine-labelled graphene oxide. Microchim Acta 186, 383 (2019). https://doi.org/10.1

• 4. Chen, S., Li, Y., Wu, S. et al. A phosphorescent probe for cephalexin consisting of mesoporous thioglycolic acid-modified Mn:ZnS quantum dots coated with a molecularly imprinted polymer. Microchim Acta 187, 40 (2020). https://doi.org/10.1007/s00604-019-403

• 3. 王文治, 楊本鵬, 馮小艷,等. 轉(zhuǎn)基因甘蔗紅糖DNA的提取及分子鑒定[J]. 分子植物育種, 2019(4):1220-1227.

• 2. 廖羽,余蘭.荷移分光光度法測(cè)定西咪替丁含量的研究[J].遵義醫(yī)科大學(xué)學(xué)報(bào),2020,43(01):104-111.

• 1. 張文靜, 黃倩倩, 陳云娜,等. 聚多巴胺包覆阿霉素/中空介孔硅納米顆粒的制備及表征[J]. 中南藥學(xué), 2020(3):364-368.

輸入產(chǎn)品批號(hào)：

本計(jì)算器可幫助您計(jì)算出特定溶液中溶質(zhì)的質(zhì)量、溶液濃度和體積之間的關(guān)系，公式為：

質(zhì)量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *