• 產(chǎn)品描述： SB 4 是一種 bone morphogenetic protein 4 (BMP4) signaling 的有效的選擇性激動(dòng)劑，其EC50值為74 nM。SB 4可增強(qiáng)經(jīng)典的BMP信號(hào)傳導(dǎo)并激活 SMAD-1/5/9 磷酸化

• 靶點(diǎn)： BMP4(Cell-free assay):74 nM(EC50);TGF-beta/Smad

• 體外研究：
  BMP signaling agonist sb4 (0.05 μM-1 μM; 24 hours) induces phosphorylated SMAD-1/5/9 in a dose-dependent manner in PRECs cells under serum-starved conitions.
  BMP signaling agonist sb4 (1 μM; 0-60 mins) enhances the efficacy of signaling at each concentration of rhBMP4 tested. This effect is most pronounced at low concentrations of rhBMP4, whereby sb4 increases BRE-luc expression 2-fold at 0.4 ng/ml of rhBMP4.

• 細(xì)胞實(shí)驗(yàn)： Cell lines: Primary mouse kidney epithelial cells (PRECs) Concentrations: 0.05 μM-1 μM Incubation Time: 0-1 h, 24 h Method: The BRE-Luc cells are stimulated with 2 ng/ml rhBMP4 for 1 h, after which the media are removed and replaced with fresh media containing either DMSO or 1 μm SB 4. p-SMAD-1/5/9 levels are then examined after 0, 5, 15, 30, 45, and 60 min of SB 4 treatment and compared with DMSO controls.

• 參考文獻(xiàn)：
  1. Bradford STJ, Ranghini EJ, Grimley E, Lee PH, Dressler GR. High-throughput screens for agonists of bone morphogenetic protein (BMP) signaling identify potent benzoxazole compounds. J Biol Chem. 2019 Mar 1;294(9):3125-3136.

• 溶解性： Soluble  in  DMSO、Ethanol

• 保存條件： -20℃

• 配置溶液濃度參考：
  

  	1mg	5mg	10mg
  1 mM	3.123 ml	15.615 ml	31.23 ml
  5 mM	0.625 ml	3.123 ml	6.246 ml
  10 mM	0.312 ml	1.562 ml	3.123 ml
  50 mM	0.062 ml	0.312 ml	0.625 ml

• 注意：部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。

輸入產(chǎn)品批號(hào)：

本計(jì)算器可幫助您計(jì)算出特定溶液中溶質(zhì)的質(zhì)量、溶液濃度和體積之間的關(guān)系，公式為：

質(zhì)量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *