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• 產(chǎn)品描述： Yoda 1 is a potent and selective Piezo1 agonist. Yoda 1 activates purified Piezo1 channels. Yoda 1 potently inhibits macropinocytosis induced by epidermal growth factor (EGF). Yoda 1 enhances Ca2+ influx followed by activation of the calcium-activated potassium channel KCa3.1 and inhibition of Rac1 activation

• 靶點(diǎn)： ERK1;ERK2;PiezoChannel

• 體外研究：
  Yoda1 (0-6 μM, 5 min) induces the activation of both Akt and ERK1/2, which is not dependent on Piezo1. Yoda1 (1.5 μM, 5 min) inhibits Rac1 activation. Western Blot Analysis Cell Line: human coronary artery endothelial cells (HCAECs)[2], A431 cells Concentration: 0, 1.5, 3.0, and 6.0?μM Incubation Time: 5 min Result: Induced the activation of both Akt and ERK1/2, and increased the phosphorylation levels of both Akt and ERK1/2 in a dose-dependent manner. Inhibited EGF-induced increase in the amount of Rac1-GTP, and inhibited Rac1 activation

• 參考文獻(xiàn)：
  1. Syeda R, et al. Chemical activation of the mechanotransduction channel Piezo1. Elife. 2015 May 22;4. 2. Dela Paz NG, et al. Yoda1-induced phosphorylation of Akt and ERK1/2 does not require Piezo1 activation. Biochem Biophys Res Commun. 2018 Feb 26;497(1):220-225. 3. Kuriyama M, et al. Piezo1 activation using Yoda1 inhibits macropinocytosis in A431 human epidermoid carcinoma cells. Sci Rep. 2022 Apr 15;12(1):6322.

• 溶解性： Soluble  in  DMSO

• 保存條件： -20℃

• 配置溶液濃度參考：
  

  	1mg	5mg	10mg
  1 mM	2.815 ml	14.074 ml	28.148 ml
  5 mM	0.563 ml	2.815 ml	5.63 ml
  10 mM	0.281 ml	1.407 ml	2.815 ml
  50 mM	0.056 ml	0.281 ml	0.563 ml

• 注意：部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。

輸入產(chǎn)品批號(hào)：

本計(jì)算器可幫助您計(jì)算出特定溶液中溶質(zhì)的質(zhì)量、溶液濃度和體積之間的關(guān)系，公式為：

質(zhì)量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *